Nestin gene expression in stromal precursor cells from the human bone marrow

INTRODUCTION: The hierarchy of stromal progenitors from the bone marrow is poorly characterized; multipotent mesenchymal cells and colony-forming units fibroblasts are isolated in culture. Mesenchymal stem do not have a unique combination surface antigens, making it difficult to obtain pure population. expression nestin gene often used as marker these cells.
 AIM: To evaluate level characterize change its during transition oligopotent progenitor monopotent ones.
 MATERIALS AND METHODS: Stromal were analyzed samples 19 donors by standard methods. A total 296 individual clones fibroblast obtained same samples. for ability differentiate toward adipogenic osteogenic lineages. Relative was all RESULTS: Mean relative did differ significantly (0.410.13) population (0.240.05). In fibroblasts, higher than (0.310.04). When analyzing differing their differentiation potential, highest found group progenitors, while lower progenitors.
 CONCLUSION: Nestin specific cannot be this cell type. According our data, high rather identifies progenitors.